Plasmid_Backbone

Part:BBa_K3841023:Design

Designed by: ktherkelsen   Group: iGEM21_DTU-Denmark   (2021-10-16)


pGGAselect Destination Plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 434
    Illegal PstI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 434
    Illegal PstI site found at 234
    Illegal NotI site found at 445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 434
    Illegal BglII site found at 356
    Illegal BamHI site found at 290
    Illegal XhoI site found at 284
    Illegal XhoI site found at 439
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 434
    Illegal PstI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 434
    Illegal PstI site found at 234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 395
    Illegal BsaI.rc site found at 320


Design Notes

pGGAselect is suitable for Golden Gate Assembly. Either digest the backbone with BsaI or BsmBI for prepare it for insert or assembly.

Source

New England Biolabs (https://international.neb.com/-/media/nebus/page-images/tools-and-resources/interactive-tools/dna-sequences-and-maps/text-documents/pggaselectfsa.txt?rev=87941bf93a1d49f28c69930c8289e15f&hash=4A71E1E81374F863510F52544BA65704)

References

https://www.neb.com/-/media/nebus/files/manuals/manuale1601.pdf